Reconstructions from cross-correlation and our alignment approach.
(a-c) Cross-section images of the 3-D volume from cross-correlation alignment. (d-f) Same cross-sections shown as (a-c) resulting from implementing our alignment method. (g, h) 3-D volume renders of the two reconstructions from cross-correlation alignment (g) and our alignment method (h). The scale bar in (a) is valid for (a-f), and the scale bar in (g) is valid for (g) and (h). It is apparent from these images that more blurring is present from the cross-correlation as a result of misalignment.