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Table 1 Dose reduction by I-DS and T-DS approaches

From: Reduced-dose and high-speed acquisition strategies for multi-dimensional electron microscopy

I-DS (% of pixels sampled)

T-DS (angular increment)

Relative dose

Resolution (FSC = 0.3) (nm)

100

2° (70 projections)

1.0

6.31

100

8° (18 projections)

0.25

11.7

60

4° (35 projections)

0.3

7.69

30

4° (35 projections)

0.15

8.21

30

6° (24 projections)

0.1

8.8

10

2° (70 projections)

0.1

8.2

  1. I-DS, image-downsampling; T-DS, tilt-downsampling; FSC, Fourier Shell Correlation. Summary of selected I-DS and T-DS settings with the corresponding relative dose and resolution of the CS-ET reconstructions (Fourier Shell Correlation (FSC) = 0.3).