Fig. 2
From: Automated analysis of evolving interfaces during in situ electron microscopy
Beam-induced Au crystal growth in the nanoaquarium with transmitted beam current of 7.8 nA spread over ~2 μm diameter, resulting in a dose rate of 5.9 × 108 (Gy/s) or 160 (e−/Å2-s). Stock solutions of chloroauric acid (Sigma-Aldrich) were made by dissolving the salt in deionized (DI) water at concentration of 20 mM. The liquid thickness within the imaged region is 50–100 nm. The left column shows de-noising: a a nanocrystal sub-image, b the same image after TV filter application, and c the difference between images a and b. The second column shows background subtraction: d the image used as the background model, e the frame of interest prior to background subtraction, and f the foreground image. The right column shows edge detection: g a thresholded foreground image (after TV filter), h the extracted edges from the foreground image in g, and i the extracted edges without image preparation (undesired)