Fig. 1From: 3D reconstruction of biological structures: automated procedures for alignment and reconstruction of multiple tilt series in electron tomographyProtocol for acquiring multiple tilt series. A Between each series, the sample is rotated within the goniometer holder (left side) following an orientational multilevel access scheme (MAS) that optimizes the reconstruction space sampling. The tilt axis is displayed schematically for the 8-tilt series case (right side); series are labeled with letters from ‘a’ to ‘h’. The first and last series ‘a’ and ‘h’ correspond to a 0°–157.5° sample orientation, respectively. B 3D illustration representing the increase in accessible volume information as the number of tilt series augmentsBack to article page