Fig. 10From: 3D reconstruction of biological structures: automated procedures for alignment and reconstruction of multiple tilt series in electron tomographyDemonstration of the gold marker detection scheme outlined in "Appendix: Marker detection." This filtering procedure makes use of gaussian derivatives to calculate the second-order derivatives and allows to selectively enhance markers of a specific size (see recipe [21]). A A projection micrograph of the clathrin specimen at zero tilt is displayed. B The corresponding detector image is shown. The biological background behind the 5-nm gold markers is largely attenuated after applying this filter.Back to article page