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Fig. 11 | Advanced Structural and Chemical Imaging

Fig. 11

From: 3D reconstruction of biological structures: automated procedures for alignment and reconstruction of multiple tilt series in electron tomography

Fig. 11

Flow chart describing the automation process for a single tilt series reconstruction. After detecting the marker projection peaks throughout the micrographs, 3D marker positions \(\{\mathbf{X^\tau }\}_\tau\) are inferred using a cluster analysis; this analysis is carried out on a set of 3D points located at the cross-over nodes of back-projected rays (Fig. 12). A trace (\(\equiv\) set of matching peaks in a micrograph series) is then estimated for each marker, and the projection maps refined with a bundle adjustment procedure. This process is repeated iteratively to maximize the average trace size \(\langle l_\tau \rangle _\tau\)

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