Clathrin-coated vesicle reconstruction using a 16 tilt series scheme. A EM snapshot at zero tilt; gold markers (G) are indicated with arrows. B
XY section in the middle of the reconstruction. Note the contrast increase between the projection in A and the tomogram slice. Free ribosomal units (R) are indicated with arrows. The diameter of this organelle inner vesicle averaged around 100 nm, making it significantly larger than previously reported similar structures . C The number of triskelions should increase linearly with the vesicle surface; we counted 143 triskelions, which is consistent with the trend reported in . Indeed, the square root of triskelions number versus inner vesicle diameter roughly follows a straight line.