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Fig. 9 | Advanced Structural and Chemical Imaging

Fig. 9

From: 3D reconstruction of biological structures: automated procedures for alignment and reconstruction of multiple tilt series in electron tomography

Fig. 9

Clathrin-coated vesicle reconstruction using different schemes. A XY tomogram slices containing a partial view of the clathrin cage obtained with different reconstruction schemes (FBP, N = 1, 2 and 16; wSIRT, N = 16). Note the free ribosomal units (R) around the cage. B, C An isosurface of a portion of the cage reconstruction (orange wireframe) is compared to the corresponding theoretical protein model assembly (solid gray surface). No extra matching transformation other than simple translations/rotations is necessary. D A segmentation of the clathrin cage is displayed on a wider scale showing a rough endoplasmic reticulum (ER) as well

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