Fig. 10From: Deceleration of probe beam by stage bias potential improves resolution of serial block-face scanning electron microscopic imagesTypical images collected on the FEI CBS detector with 1.5-keV deceleration voltage. The separation of SE signals from BSE signals is possible by turning off the inner-most ring and using only the outer rings of the FEI CBS detector. Images were collected at 3-keV gun-accelerating electron energy and −1.5-keV deceleration voltage. a Signal from the central ring only. The signal is composed of a mix of BSEs and SEs, but it is dominated by SEs. b Signal from the outer three rings of the CBS detector. The signal contains almost no SE signal. With the monolithic Gatan BSE detector, separation of the BSE and SE signals with deceleration is not possible unless the SEs are blocked. The intensity has been inverted to produce TEM-like contrastBack to article page