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Fig. 11 | Advanced Structural and Chemical Imaging

Fig. 11

From: Deceleration of probe beam by stage bias potential improves resolution of serial block-face scanning electron microscopic images

Fig. 11

10 µm × 10 µm × 4 µm volume of rodent cerebellum collected by SBEM using sample biasing. Imaging was performed at 3.0-keV column-electron energy and a landing energy of 1.5 keV. The beam current was 129 pA at the sample with a magnification of ×3200 and 5 µs dwell time per pixel. The cutting depth on this sample was 50 nm

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