Fig. 12

3D cross-section view of a 40 µm × 40 µm × 8 µm volume of rodent cerebellum collected by SBEM using 3-keV primary beam energy and a −1-keV sample bias to achieve a 2-keV landing energy. The volume was collected on a Zeiss Sigma using the standard monolithic BSE detector from Gatan and a 5-mm outer diameter and 0.8-mm inner diameter SE-blocking aperture appropriate to blocking SEs from a 3-keV primary beam, a −1-keV bias potential, and an 8.9-mm working distance. The volume was collected at 1.5K× magnification, 1.5-µs dwell time, and a 4K × 4K× raster scan at a beam current of 55 pA