Fig. 2
From: Protein structural biology using cell-free platform from wheat germ
Expression trials using PCR templates. a Schematic of two-step PCR template preparation for CellFree Sciences wheat germ expression kit followed by transcription and translation in the presence of the FluoroTect GreenLys tRNA. Elements shown are the SP6 promoter (SP6), the translational enhancer sequence (E01) and the untranslated region (UTR). SPU and deSP6E01 are internal names for the respective primers used during PCR. b The detection of PCR products on agarose. c The detection of mRNAs on agarose. d SDS-PAGE gel showing the expression of fluorophore-containing translation products. BLANK is a negative control translation which has no PCR template added to the reaction mixture