Fig. 3
From: Protein structural biology using cell-free platform from wheat germ
Protein synthesis and purification. a SDS-PAGE gel, showing the expression and purification of CCMK protein using 6×His-tag. Lane 1, control DHFR expression; lane 2, crude mixture; lane 3, soluble fraction; lane 4, flow-through; lane 5, elution fraction 1; lane 6, elution fraction 2; lane 7, elution fractions 1 and 2 combined and concentrated; lane 8, MW markers. b SDS-PAGE gel of expression and purification of GBSSI using 3XFLAG-tag. Lane 1, control DHFR expression; lane 2, crude mixture; lane 3, soluble fraction; lane 4, flow-through; lane 5, elution fraction 1; lane 6, elution fraction 2; lane 7, elution fractions 1 and 2 combined and concentrated; lane 8, 3XFLAG tag removed; lane 9, MW markers