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Fig. 5 | Advanced Structural and Chemical Imaging

Fig. 5

From: Protein structural biology using cell-free platform from wheat germ

Fig. 5

Cryo-EM applications of cell-free-synthesized protein PDX1.2. a Raw cryo-EM image of PDX1.2 particles distributed in vitreous ice and adopting random orientations. b Single-particle class averages of cryo-EM dataset from (a) showing class averages for the top, side and two intermediate views. A double stacked hexameric ring geometry is evident. c Preliminary 3D SPA cryo-EM reconstruction of PDX1.2 complex shown in three different views at 15 Å resolution. All scale bars correspond to 20 nm

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