Fig. 2From: Investigation of hole-free phase plate performance in transmission electron microscopy under different operation conditions by experiments and simulationsThon ring patterns of images of an amorphous carbon sample taken with an HFPP operated under case A, B, C conditions and corresponding phase shift profiles \(\varphi_{\text{PP}} \left( q \right)\). a Case A (small ZOB diameter ~ 100 nm, unheated HFPP) with experimental Thon ring pattern and PCTF fit after 30 s of HFPP illumination. Fit parameters are \(\varphi_{\text{PP}} \left( 0 \right) = \left( {2.76 \pm 0.06} \right) \pi\) and \(\Delta f = \left( { - 9.63 \pm 0.12} \right)\) µm. Minima and maxima of the experimental Thon ring pattern marked by red and green lines do not show distinct deviations between experimental data and fit. b Case B (small ZOB, HFPP temperature 200 °C) with experimental Thon ring pattern and PCTF fit after 150 s of illumination (upper graph) with \(\varphi_{\text{PP}} \left( 0 \right) = \left( { - 0.82 \pm 0.05} \right) \pi\) and \(\Delta f = \left( { - 0.65 \pm 0.13} \right)\) µm. Pronounced differences occur for minima and maxima of the experimental Thon ring pattern and the PCTF fit at small \(q\). The extended phase shift profile \(\varphi_{\text{PP}} \left( q \right)\) (lower graph) was calculated as the difference between the argument of the fitted PCTF and the experimental Thon ring pattern. c Case C (large ZOB ~ 250 nm, unheated HFPP) with experimental Thon ring pattern and PCTF fit (upper graph) after 150 s of illumination with \(\varphi_{\text{PP}} \left( 0 \right) = \left( { - 3.55 \pm 0.11} \right) \pi\) and \(\Delta f = \left( { - 1.73 \pm 0.03} \right)\) µm. Pronounced differences occur for minima and maxima of the experimental Thon ring pattern and PCTF at small \(q\). The extended phase shift profile \(\varphi_{\text{PP}} \left( q \right)\) (lower graph) was calculated as the difference between the argument of the fitted PCTF and the experimental Thon ring patternBack to article page