Fig. 3

Preservation of YFP fluorescence after resin embedding. a Wide-field fluorescence microscopy of live leaf tissue showing multiple FtsZ2–mYFP mini-rings per chloroplast. b Semi-thin section from aldehyde-fixed tissue embedded in LR White. Chloroplasts are recognized by their high fluorescence background. c–d Semi-thin sections from leaf tissue subjected to HPF–FS and embedding in LR White. Chloroplasts are delineated by dotted lines. c Punctate and short linear FtsZ2–mYFP assemblies in resin sections. The area corresponding to the highlighted square is shown at higher magnification in the insert where some of the assemblies are resolved as mini-rings (arrows). d Mutant plant lacking FtsZ2 proteins (negative control). Scale bars represent 5 µm in a–d and 2 µm in the insert in c